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Biology & Biomedical Research

FastQC

Open-source software for checking raw high-throughput sequencing data and producing modular plots, tables, and HTML reports.

Last verified

RESEARCH USE

Where FastQC fits

FastQC provides a rapid diagnostic view of raw sequencing data before alignment, quantification, or variant analysis. It reports measures such as base-quality patterns, sequence composition, duplication, and adapter content in modular HTML output. Interpret flags in the context of the library protocol and sequencing platform, and retain the software version, settings, and original reports.

Research tasks

  • Inspect quality patterns in raw sequencing reads
  • Flag possible adapter, duplication, and composition issues
  • Generate archivable QC reports for sequencing pipelines

What to evaluate before use

  • Warning and failure flags use general thresholds and do not by themselves make a sample unusable.
  • FastQC does not trim, align, or correct reads. Any intervention should follow the experimental design and downstream requirements.

Verification note

This entry summarizes the tool's role without assessing scientific accuracy or endorsing its outputs. Features and terms can change; consult the official source before adopting it for consequential work.

sequencing QCFASTQ

Last verified: 2026-09-04
Source: official documentation ↗