Biology & Biomedical Research

SeqKit

A cross-platform open-source command-line toolkit for querying, transforming, summarizing, sampling, and indexing FASTA and FASTQ files.

Last verified

RESEARCH USE

Where SeqKit fits

SeqKit supports scripted inspection and transformation of FASTA and FASTQ files, including statistics, search, conversion, sampling, duplicate removal, and subsequence extraction. Record input checksums, sequence type, patterns or coordinate rules, random seeds, output-order choices, and the SeqKit version.

Research tasks

  • Summarize and inspect FASTA or FASTQ files
  • Filter, transform, sample, or deduplicate sequence records
  • Extract subsequences by identifiers or coordinates

What to evaluate before use

  • Some commands infer sequence type from the beginning of a file; mixed alphabets or unusual symbols require an explicit check of the alphabet setting.
  • Identifier rewriting, deduplication, sampling, and format conversion can disrupt pairing or provenance unless retention rules are defined in advance.

Verification note

This entry summarizes the tool's role without assessing scientific accuracy or endorsing its outputs. Features and terms can change; consult the official source before adopting it for consequential work.

sequence filesFASTA/FASTQ

Last verified: 2026-09-07
Source: official documentation ↗